This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
2cjt
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="2cjt" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cjt, resolution 1.44Å" /> '''STRUCTURAL BASIS FO...)
Next diff →
Revision as of 18:55, 29 October 2007
|
STRUCTURAL BASIS FOR A MUNC13-1 HOMODIMER- MUNC13-1- RIM HETERODIMER SWITCH: C2-DOMAINS AS VERSATILE PROTEIN-PROTEIN INTERACTION MODULES
Overview
C(2) domains are well characterized as Ca(2+)/phospholipid-binding, modules, but little is known about how they mediate protein-protein, interactions. In neurons, a Munc13-1 C(2)A-domain/RIM zinc-finger domain, (ZF) heterodimer couples synaptic vesicle priming to presynaptic, plasticity. We now show that the Munc13-1 C(2)A domain homodimerizes, and, that homodimerization competes with Munc13-1/RIM heterodimerization. X-ray, diffraction studies guided by nuclear magnetic resonance (NMR) experiments, reveal the crystal structures of the Munc13-1 C(2)A-domain homodimer and, the Munc13-1 C(2)A-domain/RIM ZF heterodimer at 1.44 A and 1.78 A, resolution, respectively. The C(2)A domain adopts a beta-sandwich, structure with a four-stranded concave side that mediates, homodimerization, leading to ... [(full description)]
About this Structure
2CJT is a [Single protein] structure of sequence from [Rattus norvegicus] with EDO and FMT as [ligands]. Full crystallographic information is available from [OCA].
Reference
Structural basis for a Munc13-1 homodimer to Munc13-1/RIM heterodimer switch., Lu J, Machius M, Dulubova I, Dai H, Sudhof TC, Tomchick DR, Rizo J, PLoS Biol. 2006 Jun;4(7):e192. PMID:16732694
Page seeded by OCA on Mon Oct 29 20:59:58 2007
Categories: Rattus norvegicus | Single protein | Dai, H. | Dulubova, I. | Lu, J. | Machius, M. | Rizo, J. | Sudhof, T.C. | Tomchick, D.R. | EDO | FMT | Alternative splicing | C2 domains | Coiled coil | Exocytosis | Metal-binding | Munc13 | Neurotransmitter release | Phorbol-ester binding | Protein-protein interactions | Rim | Synaptosome | Zinc | Zinc finger
