1s2j
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(New page: 200px<br /><applet load="1s2j" size="450" color="white" frame="true" align="right" spinBox="true" caption="1s2j, resolution 2.20Å" /> '''Crystal structure of...)
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Revision as of 23:55, 20 November 2007
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Crystal structure of the Drosophila pattern-recognition receptor PGRP-SA
Overview
The Drosophila peptidoglycan recognition protein SA (PGRP-SA) is, critically involved in sensing bacterial infection and activating the Toll, signaling pathway, which induces the expression of specific antimicrobial, peptide genes. We have determined the crystal structure of PGRP-SA to, 2.2-A resolution and analyzed its peptidoglycan (PG) recognition and, signaling activities. We found an extended surface groove in the structure, of PGRP-SA, lined with residues that are highly diverse among different, PGRPs. Mutational analysis identified it as a PG docking groove required, for Toll signaling and showed that residue Ser158 is essential for both PG, binding and Toll activation. Contrary to the general belief that PGRP-SA, has lost enzyme function and serves primarily for PG sensing, we found, that it possesses an intrinsic L,D-carboxypeptidase activity for, diaminopimelic acid-type tetrapeptide PG fragments but not lysine-type PG, fragments, and that Ser158 and His42 may participate in the hydrolytic, activity. As L,D-configured peptide bonds exist only in prokaryotes, this, work reveals a rare enzymatic activity in a eukaryotic protein known for, sensing bacteria and provides a possible explanation of how PGRP-SA, mediates Toll activation specifically in response to lysine-type PG.
About this Structure
1S2J is a Single protein structure of sequence from Drosophila melanogaster with PO4 as ligand. Active as Muramoyltetrapeptide carboxypeptidase, with EC number 3.4.17.13 Full crystallographic information is available from OCA.
Reference
A Drosophila pattern recognition receptor contains a peptidoglycan docking groove and unusual L,D-carboxypeptidase activity., Chang CI, Pili-Floury S, Herve M, Parquet C, Chelliah Y, Lemaitre B, Mengin-Lecreulx D, Deisenhofer J, PLoS Biol. 2004 Sep;2(9):E277. Epub 2004 Sep 7. PMID:15361936
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