1sjj
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(New page: 200px<br /><applet load="1sjj" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sjj" /> '''Cryo-EM Structure of Chicken Gizzard Smooth ...)
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Revision as of 00:18, 21 November 2007
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Cryo-EM Structure of Chicken Gizzard Smooth Muscle alpha-Actinin
Overview
Cryoelectron microscopy was used to obtain a 3-D image at 2.0 nm, resolution of 2-D arrays of smooth muscle alpha-actinin. The, reconstruction reveals a well-resolved long central domain with 90 degrees, of left-handed twist and near 2-fold symmetry. However, the molecular ends, which contain the actin binding and calmodulin-like domains, have, different structures oriented approximately 90 degrees to each other., Atomic structures for the alpha-actinin domains were built by homology, modeling and assembled into an atomic model. Model building suggests that, in the 2-D arrays, the two calponin homology domains that comprise the, actin-binding domain have a closed conformation at one end and an open, conformation at the other end due to domain swapping. The open and closed, conformations of the actin-binding domain suggests flexibility that may, underlie Ca2+ regulation. The approximately 90 degrees orientation, difference at the molecular ends may underlie alpha-actinin's ability to, crosslink actin filaments in nearly any orientation.
About this Structure
1SJJ is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
A 3-D reconstruction of smooth muscle alpha-actinin by CryoEm reveals two different conformations at the actin-binding region., Liu J, Taylor DW, Taylor KA, J Mol Biol. 2004 Apr 16;338(1):115-25. PMID:15050827
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