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1sq9

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(New page: 200px<br /><applet load="1sq9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sq9, resolution 1.9&Aring;" /> '''Structure of Ski8p, a...)
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Revision as of 00:26, 21 November 2007

Image:1sq9.jpg

1sq9, resolution 1.9Å

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Structure of Ski8p, a WD repeat protein involved in mRNA degradation and meiotic recombination

Overview

Ski8p is a 44-kD protein that primarily functions in the regulation of, exosome-mediated, 3'--> 5' degradation of damaged mRNA. It does so by, forming a complex with two partner proteins, Ski2p and Ski3p, which, complete a complex that is capable of recruiting and activating the, exosome/Ski7p complex that functions in RNA degradation. Ski8p also, functions in meiotic recombination in complex with Spo11 in yeast. It is, one of the many hundreds of primarily eukaryotic proteins containing, tandem copies of WD repeats (also known as WD40 or beta-transducin, repeats), which are short ~40 amino acid motifs, often terminating in a, Trp-Asp dipeptide. Genomic analyses have demonstrated that WD repeats are, found in 1%-2% of proteins in a typical eukaryote, but are extremely rare, in prokaryotes. Almost all structurally characterized WD-repeat proteins, are composed of seven such repeats and fold into seven-bladed beta, propellers. Ski8p was thought to contain five WD repeats on the basis of, primary sequence analysis implying a five-bladed propeller. The 1.9 A, crystal structure unexpectedly exhibits a seven-bladed propeller fold with, seven structurally authentic WD repeats. Structure-based sequence, alignments show additional sequence diversity in the two undetected, repeats. This demonstrates that many WD repeats have not yet been, identified in sequences and also raises the possibility that the, seven-bladed propeller may be the predominant fold for this family of, proteins.

About this Structure

1SQ9 is a Single protein structure of sequence from Saccharomyces cerevisiae with SO4 as ligand. Full crystallographic information is available from OCA.

Reference

The structure of Ski8p, a protein regulating mRNA degradation: Implications for WD protein structure., Madrona AY, Wilson DK, Protein Sci. 2004 Jun;13(6):1557-65. PMID:15152089

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