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2gzw

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{{STRUCTURE_2gzw| PDB=2gzw | SCENE= }}
{{STRUCTURE_2gzw| PDB=2gzw | SCENE= }}
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'''Crystal structure of the E.coli CRP-cAMP complex'''
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===Crystal structure of the E.coli CRP-cAMP complex===
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==Overview==
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The structure of a dimer of the Escherichia coli catabolite gene activator protein has been refined at 2.5 A resolution to a crystallographic R-factor of 20.7% starting with coordinates fitted to the map at 2.9 A resolution. The two subunits are in different conformations and each contains one bound molecule of the allosteric activator, cyclic AMP. The amino-terminal domain is linked to the smaller carboxy-terminal domain by a nine-residue hinge region that exists in different conformations in the two subunits, giving rise to approximately a 30 degree rotation between the positions of the small domains relative to the larger domains. The amino-terminal domain contains an antiparallel beta-roll structure in which the interstrand hydrogen bonding is well-determined. The beta-roll can be described as a long antiparallel beta-ribbon that folds into a right-handed supercoil and forms part of the cyclic AMP binding site. Each cyclic AMP molecule is in an anti conformation and has ionic and hydrogen bond interactions with both subunits.
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The line below this paragraph, {{ABSTRACT_PUBMED_2828639}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 2828639 is the PubMed ID number.
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{{ABSTRACT_PUBMED_2828639}}
==About this Structure==
==About this Structure==
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[[Category: Structural genomic]]
[[Category: Structural genomic]]
[[Category: Transcription]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 05:42:06 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Sep 29 07:55:50 2008''

Revision as of 04:55, 29 September 2008

Image:2gzw.png

Template:STRUCTURE 2gzw

Crystal structure of the E.coli CRP-cAMP complex

Template:ABSTRACT PUBMED 2828639

About this Structure

2GZW is a Single protein structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.

Reference

Structure of a complex of catabolite gene activator protein and cyclic AMP refined at 2.5 A resolution., Weber IT, Steitz TA, J Mol Biol. 1987 Nov 20;198(2):311-26. PMID:2828639

Page seeded by OCA on Mon Sep 29 07:55:50 2008

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