1sxd
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(New page: 200px<br /><applet load="1sxd" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sxd" /> '''Solution Structure of the Pointed (PNT) Doma...)
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Revision as of 00:41, 21 November 2007
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Solution Structure of the Pointed (PNT) Domain from mGABPa
Overview
The PNT (or Pointed) domain, present within a subset of the Ets family of, transcription factors, is structurally related to the larger group of SAM, domains through a common tertiary arrangement of four alpha-helices., Previous studies have shown that, in contrast to the PNT domain from Tel, this domain from Ets-1 contains an additional N-terminal helix integral to, its folded structure. To further investigate the structural plasticity of, the PNT domain, we have used NMR spectroscopy to characterize this domain, from two additional Ets proteins, Erg and GABPalpha. These studies both, define the conserved and variable features of the PNT domain, and, demonstrate that the additional N-terminal helix is also present in, GABPalpha, but not Erg. In contrast to Tel and Yan, which self-associate, to form insoluble polymers, we also show that the isolated PNT domains, from Ets-1, Ets-2, Erg, Fli-1, GABPalpha, and Pnt-P2 are monomeric in, solution. Furthermore, these soluble PNT domains do not associate in any, pair-wise combination. Thus these latter Ets family PNT domains likely, mediate interactions with additional components of the cellular signaling, or transcriptional machinery.
About this Structure
1SXD is a Single protein structure of sequence from Mus musculus. Full crystallographic information is available from OCA.
Reference
Diversity in structure and function of the Ets family PNT domains., Mackereth CD, Scharpf M, Gentile LN, MacIntosh SE, Slupsky CM, McIntosh LP, J Mol Biol. 2004 Sep 24;342(4):1249-64. PMID:15351649
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