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1sxl
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(New page: 200px<br /><applet load="1sxl" size="450" color="white" frame="true" align="right" spinBox="true" caption="1sxl" /> '''RESONANCE ASSIGNMENTS AND SOLUTION STRUCTURE...)
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Revision as of 00:42, 21 November 2007
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RESONANCE ASSIGNMENTS AND SOLUTION STRUCTURE OF THE SECOND RNA-BINDING DOMAIN OF SEX-LETHAL DETERMINED BY MULTIDIMENSIONAL HETERONUCLEAR MAGNETIC RESONANCE SPECTROSCOPY
Overview
The RNA-binding protein Sex-lethal (Sxl) is a critical regulator of sexual, differentiation and dosage compensation in Drosophila. This regulatory, activity is a consequence of the ability of Sxl to bind uridine-rich RNA, tracts involved in pre-mRNA splicing. Sxl contains two RNP consensus-type, RNA-binding domains (RBDs). A structural study of a portion of Sxl (amino, acids 199-294) containing the second RNA-binding domain (RBD-2) using, multidimensional heteronuclear NMR is presented here. Nearly complete 1H, 13C, and 15N resonance assignments have been obtained from 15N- and, 13C/15N-uniformly labeled protein. These assignments were used to analyze, 3D 15N-separated NOESY and 13C/13C-separated 4D NOESY spectra which, produced 494 total and 169 long-range NOE-derived distance restraints., Along with 41 backbone dihedral restraints, these distance restraints were, employed to generate an intermediate-resolution family of calculated, structures, which exhibits the beta alpha beta-beta alpha beta tertiary, fold found in other RBD-containing proteins. The RMSD to the average, structure for the backbone atoms of residues 11-93 is 1.55 +/- 0.30 A, while the RMSD for backbone atoms involved in secondary structure is 0.76, +/- 0.14 A. A capping box [Harper, E.T., & Rose, G.D. (1993) Biochemistry, 32, 7605-7609] was identified at the N-terminus of the first helix and has, been characterized by short- and medium-range NOEs. Finally, significant, structural similarities and differences between Sxl RBD-2 and other, RBD-containing proteins are discussed.
About this Structure
1SXL is a Single protein structure of sequence from Drosophila melanogaster. Full crystallographic information is available from OCA.
Reference
Resonance assignments and solution structure of the second RNA-binding domain of sex-lethal determined by multidimensional heteronuclear magnetic resonance., Lee AL, Kanaar R, Rio DC, Wemmer DE, Biochemistry. 1994 Nov 22;33(46):13775-86. PMID:7524663
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