1sym

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3-D SOLUTION STRUCTURE OF REDUCED APO-S100B FROM RAT, NMR, 20 STRUCTURES

Overview

S100B(beta beta), a member of the S100 protein family, is a Ca(2+)-binding, protein with noncovalent interactions at its dimer interface. Each, apo-S100 beta subunit (91 residues) has four alpha-helices and a small, antiparallel beta-sheet, consistent with two predicted helix-loop-helix, Ca(2+)-binding domains known as EF-hands [Amburgey et al. (1995) J., Biomol. NMR 6, 171-179]. The three-dimensional solution structure of, apo-S100B(beta beta) from rat has been determined using 2672 distance, (14.7 per residue) and 88 dihedral angle restraints derived from, multidimensional nuclear magnetic resonance spectroscopy. Apo-S100B (beta, beta) is found to be globular and compact with an extensive hydrophobic, core and a highly charged surface, consistent with its high solubility. At, the symmetric dimer interface, 172 intermolecular nuclear Overhauser, effect correlations (NOEs) define the antiparallel alignment of helix I, with I' and of helix IV with IV'. The perpendicular association of these, pairs of antiparallel helices forms an X-type four-helical bundle at the, dimer interface. Whereas, the four helices within each apo-S100 beta, subunit adopt a unicornate-type four-helix bundle, with helix I protruding, from the parallel bundle of helices II, III, and IV. Accordingly, the, orientation of helix III relative to helices I, II, and IV in each subunit, differs significantly from that known for other Ca(2+)-binding proteins., Indeed, the interhelical angle (omega) observed in the C-terminal EF-hand, of apo-S100 beta is -142 degrees, whereas omega ranges from 118 degrees to, 145 degrees in the apo state and from 84 degrees to 128 degrees in the, Ca(2+)-bound state for the EF-hands of calbindin D9k, calcyclin, and, calmodulin. Thus, a significant conformational change in the C-terminal, EF-hand would be required for it to adopt a structure typical of the, Ca(2+)-bound state, which could readily explain the dramatic spectral, effects observed upon the addition of Ca2+ to apo-S100B(beta beta).

About this Structure

1SYM is a Single protein structure of sequence from Rattus norvegicus. Full crystallographic information is available from OCA.

Reference

Solution structure of rat apo-S100B(beta beta) as determined by NMR spectroscopy., Drohat AC, Amburgey JC, Abildgaard F, Starich MR, Baldisseri D, Weber DJ, Biochemistry. 1996 Sep 10;35(36):11577-88. PMID:8794737

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