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1gim
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(New page: 200px<br /> <applet load="1gim" size="450" color="white" frame="true" align="right" spinBox="true" caption="1gim, resolution 2.5Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 19:02, 29 October 2007
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CRYSTAL STRUCTURE OF ADENYLOSUCCINATE SYNTHETASE FROM ESCHERICHIA COLI COMPLEXED WITH GDP, IMP, HADACIDIN, NO3-, AND MG2+. DATA COLLECTED AT 100K (PH 6.5)
Overview
Crystal structures of adenylosuccinate synthetase from Esherichia coli, complexed with Mg2+, IMP, GDP, NO3- and hadacidin at 298 and 100 K have, been refined to R-factors of 0.188 and 0.206 against data to 2.8 A and 2.5, A resolution, respectively. Conformational changes of up to 9 A relative, to the unligated enzyme occur in loops that bind to Mg2+, GDP, IMP and, hadacidin. Mg2+ binds directly to GDP, NO3-, hadacidin and the protein, but is only five-coordinated. Asp13, which approaches, but does not occupy, the sixth coordination site of Mg2+, hydrogen bonds to N1 of IMP. The, nitrogen atom of NO3- is approximately 2.7 A from O6 of IMP, reflecting a, strong electrostatic interaction between the electron-deficient nitrogen, atom and the electron-rich O6. The spatial relationships between ... [(full description)]
About this Structure
1GIM is a [Single protein] structure of sequence from [Escherichia coli] with MG, NO3, HAD, IMP and GDP as [ligands]. Active as [[1]], with EC number [6.3.4.4]. Full crystallographic information is available from [OCA].
Reference
Crystal structures of adenylosuccinate synthetase from Escherichia coli complexed with GDP, IMP hadacidin, NO3-, and Mg2+., Poland BW, Fromm HJ, Honzatko RB, J Mol Biol. 1996 Dec 20;264(5):1013-27. PMID:9000627
Page seeded by OCA on Mon Oct 29 21:06:41 2007
