This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1tjf
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1tjf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tjf, resolution 2.21Å" /> '''The crystal structur...)
Next diff →
Revision as of 01:12, 21 November 2007
|
The crystal structure of the N-terminal domain of CAP indicates variable oligomerisation
Overview
Cyclase-associated protein (CAP) is a highly conserved and widely, distributed protein that links the nutritional response signaling to, cytoskeleton remodeling. In yeast, CAP is a component of the adenylyl, cyclase complex and helps to activate the Ras-mediated catalytic cycle of, the cyclase. While the N-terminal domain of CAP (N-CAP) provides a binding, site for adenylyl cyclase, the C-terminal domain (C-CAP) possesses actin, binding activity. Our attempts to crystallize full-length recombinant CAP, from Dictyostelium discoideum resulted in growth of orthorhombic crystals, containing only the N-terminal domain (residues 42-227) due to, auto-proteolytic cleavage. The structure was solved by molecular, replacement with data at 2.2 A resolution. The present crystal structure, allows the characterization of a head-to-tail N-CAP dimer in the, asymmetric unit and a crystallographic side-to-side dimer. Comparison with, previously published structures of N-CAP reveals variable modes of, dimerization of this domain, but the presence of a common interface for, the side-to-side dimer.
About this Structure
1TJF is a Single protein structure of sequence from Dictyostelium discoideum with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Structural evidence for variable oligomerization of the N-terminal domain of cyclase-associated protein (CAP)., Yusof AM, Hu NJ, Wlodawer A, Hofmann A, Proteins. 2005 Feb 1;58(2):255-62. PMID:15558566
Page seeded by OCA on Wed Nov 21 03:19:19 2007
