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1tku
From Proteopedia
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(New page: 200px<br /><applet load="1tku" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tku, resolution 1.66Å" /> '''Crystal Structure of...)
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Revision as of 01:14, 21 November 2007
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Crystal Structure of 3,4-Dihydroxy-2-butanone 4-phosphate Synthase of Candida albicans in complex with Ribulose-5-phosphate
Overview
A synthetic gene specifying a putative 3,4-dihydroxy-2-butanone, 4-phosphate synthase of Candida albicans directed the synthesis of a 22.5, kDa peptide in a recombinant Escherichia coli strain. The recombinant, protein was purified to apparent homogeneity by two chromatographic steps, and was shown to catalyze the formation of L-3,4-dihydroxy-2-butanone, 4-phosphate from ribulose 5-phosphate at a rate of 332 nmol mg(-1), min(-1). Hydrodynamic studies indicated a native molecular mass of 41 kDa, in line with a homodimer structure. The protein was crystallized in its, apoform. Soaking yielded crystals in complex with the substrate ribulose, 5-phosphate. The structures were solved at resolutions of 1.6 and 1.7, angstroms, respectively, using 3,4-dihydroxy-2-butanone 4-phosphate, synthase of E. coli for molecular replacement. Structural comparison with, the orthologs of Magnaporthe grisea and Methanococcus jannaschii revealed, a hitherto unknown conformation of the essential acidic active-site loop.
About this Structure
1TKU is a Single protein structure of sequence from Candida albicans sc5314 with 5RP as ligand. Full crystallographic information is available from OCA.
Reference
Potential anti-infective targets in pathogenic yeasts: structure and properties of 3,4-dihydroxy-2-butanone 4-phosphate synthase of Candida albicans., Echt S, Bauer S, Steinbacher S, Huber R, Bacher A, Fischer M, J Mol Biol. 2004 Aug 20;341(4):1085-96. PMID:15328619
Page seeded by OCA on Wed Nov 21 03:21:44 2007
