2oky

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[[Image:2oky.jpg|left|200px]]
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{{STRUCTURE_2oky| PDB=2oky | SCENE= }}
{{STRUCTURE_2oky| PDB=2oky | SCENE= }}
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'''A non-invasive GFP-based biosensor for mercury ions'''
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===A non-invasive GFP-based biosensor for mercury ions===
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==Overview==
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Atomic resolution structures of proteins indicate that the core is typically well packed, suggesting a densely connected network of interactions between amino acid residues. The combinatorial complexity of energetic interactions in such a network could be enormous, a problem that limits our ability to relate structure and function. Here, we report a case study of the complexity of amino acid interactions in a localized region within the core of the GFP, a particularly stable and tightly packed molecule. Mutations at three sites within the chromophore-binding pocket display an overlapping pattern of conformational change and are thermodynamically coupled, seemingly consistent with the dense network model. However, crystallographic and energetic analyses of coupling between mutations paint a different picture; pairs of mutations couple through independent "hotspots" in the region of structural overlap. The data indicate that, even in highly stable proteins, the core contains sufficient plasticity in packing to uncouple high-order energetic interactions of residues, a property that is likely general in proteins.
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The line below this paragraph, {{ABSTRACT_PUBMED_18305194}}, adds the Publication Abstract to the page
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(as it appears on PubMed at http://www.pubmed.gov), where 18305194 is the PubMed ID number.
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{{ABSTRACT_PUBMED_18305194}}
==About this Structure==
==About this Structure==
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==Reference==
==Reference==
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Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake., Chapleau RR, Blomberg R, Ford PC, Sagermann M, Protein Sci. 2008 Apr;17(4):614-22. Epub 2008 Feb 27. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/18305194 18305194]
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Local complexity of amino acid interactions in a protein core., Jain RK, Ranganathan R, Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):111-6. Epub 2003 Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684834 14684834]
Local complexity of amino acid interactions in a protein core., Jain RK, Ranganathan R, Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):111-6. Epub 2003 Dec 18. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/14684834 14684834]
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Crystal structure of the Aequorea victoria green fluorescent protein., Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science. 1996 Sep 6;273(5280):1392-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703075 8703075]
[[Category: Aequorea victoria]]
[[Category: Aequorea victoria]]
[[Category: Single protein]]
[[Category: Single protein]]
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[[Category: Chapleau, R.]]
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[[Category: Chapleau, R R.]]
[[Category: Sagermann, M.]]
[[Category: Sagermann, M.]]
[[Category: Gfp]]
[[Category: Gfp]]
[[Category: Luminescent protein]]
[[Category: Luminescent protein]]
[[Category: Mercury sensor]]
[[Category: Mercury sensor]]
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Sun May 4 11:07:46 2008''
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''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Wed Nov 5 10:39:39 2008''

Revision as of 08:39, 5 November 2008

Image:2oky.png

Template:STRUCTURE 2oky

A non-invasive GFP-based biosensor for mercury ions

Template:ABSTRACT PUBMED 18305194

About this Structure

2OKY is a Single protein structure of sequence from Aequorea victoria. Full crystallographic information is available from OCA.

Reference

Design of a highly specific and noninvasive biosensor suitable for real-time in vivo imaging of mercury (II) uptake., Chapleau RR, Blomberg R, Ford PC, Sagermann M, Protein Sci. 2008 Apr;17(4):614-22. Epub 2008 Feb 27. PMID:18305194

Local complexity of amino acid interactions in a protein core., Jain RK, Ranganathan R, Proc Natl Acad Sci U S A. 2004 Jan 6;101(1):111-6. Epub 2003 Dec 18. PMID:14684834

Crystal structure of the Aequorea victoria green fluorescent protein., Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075

Page seeded by OCA on Wed Nov 5 10:39:39 2008

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