1tol
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(New page: 200px<br /><applet load="1tol" size="450" color="white" frame="true" align="right" spinBox="true" caption="1tol, resolution 1.85Å" /> '''FUSION OF N-TERMINAL...)
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Revision as of 01:20, 21 November 2007
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FUSION OF N-TERMINAL DOMAIN OF THE MINOR COAT PROTEIN FROM GENE III IN PHAGE M13, AND C-TERMINAL DOMAIN OF E. COLI PROTEIN-TOLA
Overview
BACKGROUND: Infection of male Escherichia coli cells by filamentous Ff, bacteriophages (M13, fd, and f1) involves interaction of the phage minor, coat gene 3 protein (g3p) with the bacterial F pilus (primary receptor), and subsequently with the integral membrane protein TolA (coreceptor). G3p, consists of three domains (N1, N2, and CT). The N2 domain interacts with, the F pilus, whereas the N1 domain--connected to N2 by a flexible, glycine-rich linker and tightly interacting with it on the phage--forms a, complex with the C-terminal domain of TolA at later stages of the, infection process. RESULTS: The crystal structure of the complex between, g3p N1 and TolA D3 was obtained by fusing these domains with a long, flexible linker, which was not visible in the structure, indicating its, very high disorder and presumably a lack of interference with the, formation of the complex. The interface between both domains, corresponding to approximately 1768 A2 of buried molecular surface, is, clearly defined. Despite the lack of topological similarity between TolA, D3 and g3p N2, both domains interact with the same region of the g3p N1, domain. The fold of TolA D3 is not similar to any previously known protein, motifs. CONCLUSIONS: The structure of the fusion protein presented here, clearly shows that, during the infection process, the g3p N2 domain is, displaced by the TolA D3 domain. The folds of g3p N2 and TolA D3 are, entirely different, leading to distinctive interdomain contacts observed, in their complexes with g3p N1. We can now also explain how the, interactions between the g3p N2 domain and the F pilus enable the g3p N1, domain to form a complex with TolA.
About this Structure
1TOL is a Single protein structure of sequence from Bacteriophage m13 and escherichia coli. Full crystallographic information is available from OCA.
Reference
Filamentous phage infection: crystal structure of g3p in complex with its coreceptor, the C-terminal domain of TolA., Lubkowski J, Hennecke F, Pluckthun A, Wlodawer A, Structure. 1999 Jun 15;7(6):711-22. PMID:10404600
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