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1u90
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(New page: 200px<br /><applet load="1u90" size="450" color="white" frame="true" align="right" spinBox="true" caption="1u90, resolution 2.00Å" /> '''Crystal structures o...)
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Revision as of 01:48, 21 November 2007
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Crystal structures of Ral-GppNHp and Ral-GDP reveal two novel binding sites that are also present in Ras and Rap
Overview
RalA is a GTPase with effectors such as Sec5 and Exo84 in the exocyst, complex and RalBP1, a GAP for Rho proteins. We report the crystal, structures of Ral-GppNHp and Ral-GDP. Disordered switch I and switch II, located away from crystal contacts, are observed in one of the molecules, in the asymmetric unit of the Ral-GppNHp structure. In the other molecule, in the asymmetric unit, a second Mg(2+) ion is bound to the GppNHp, gamma-phosphate in an environment in which switch I is pulled away from, the nucleotide and switch II is found in a tight beta turn. Clustering of, conserved residues on the surface of Ral-GppNHp identifies two putative, sites for protein-protein interaction. One site is adjacent to switch I., The other is modulated by switch II and is obstructed in Ral-GDP. The Ral, structures are discussed in the context of the published structures of the, Ral/Sec5 complex, Ras, and Rap.
About this Structure
1U90 is a Single protein structure of sequence from Saguinus oedipus with MG and GDP as ligands. Full crystallographic information is available from OCA.
Reference
Crystal structures of Ral-GppNHp and Ral-GDP reveal two binding sites that are also present in Ras and Rap., Nicely NI, Kosak J, de Serrano V, Mattos C, Structure. 2004 Nov;12(11):2025-36. PMID:15530367
Page seeded by OCA on Wed Nov 21 03:56:02 2007
Categories: Saguinus oedipus | Single protein | Kosak, J. | Mattos, C. | Nicely, N.I. | Serrano, V.de. | GDP | MG | Gdp | Gmppnp | Gnp | Gppnhp | Gtp | Gtpase | Ral | Ras
