1ucu
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(New page: 200px<br /><applet load="1ucu" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ucu, resolution 4.0Å" /> '''R-type straight flage...)
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Revision as of 01:54, 21 November 2007
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R-type straight flagellar filament made of full-length flagellin
Overview
The bacterial flagellar filament is a helical propeller for bacterial, locomotion. It is a helical assembly of a single protein, flagellin, and, its tubular structure is formed by 11 protofilaments in two distinct, conformations, L- and R-type, for supercoiling. The X-ray crystal, structure of a flagellin fragment lacking about 100 terminal residues, revealed the protofilament structure, but the full filament structure is, still essential for understanding the mechanism of supercoiling and, polymerization. Here we report a complete atomic model of the R-type, filament by electron cryomicroscopy. A density map obtained from image, data up to 4 A resolution shows the feature of alpha-helical backbone and, some large side chains. The atomic model built on the map reveals, intricate molecular packing and an alpha-helical coiled coil formed by the, terminal chains in the inner core of the filament, with its intersubunit, hydrophobic interactions having an important role in stabilizing the, filament.
About this Structure
1UCU is a Single protein structure of sequence from Salmonella typhimurium. Full crystallographic information is available from OCA.
Reference
Complete atomic model of the bacterial flagellar filament by electron cryomicroscopy., Yonekura K, Maki-Yonekura S, Namba K, Nature. 2003 Aug 7;424(6949):643-50. PMID:12904785
Page seeded by OCA on Wed Nov 21 04:01:37 2007
