1udi
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(New page: 200px<br /><applet load="1udi" size="450" color="white" frame="true" align="right" spinBox="true" caption="1udi, resolution 2.7Å" /> '''NUCLEOTIDE MIMICRY IN...)
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Revision as of 01:55, 21 November 2007
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NUCLEOTIDE MIMICRY IN THE CRYSTAL STRUCTURE OF THE URACIL-DNA GLYCOSYLASE-URACIL GLYCOSYLASE INHIBITOR PROTEIN COMPLEX
Overview
The Bacillus subtilis bacteriophages PBS-1 and PBS-2 protect their, uracil-containing DNA by expressing an inhibitor protein (UGI) which, inactivates the host uracil-DNA glycosylase (UDGase) base-excision repair, enzyme. Also, PBS1/2 UGI efficiently inactivates UDGases from other, biological sources, including the enzyme from herpes simplex virus type-1, (HSV-1). The crystal structure of the HSV-1 UDGase-PBS1 UGI complex at 2.7, angstrum reveals an alpha-beta-alpha sandwich structure for UGI which, interacts with conserved regions of UDGase involved in DNA binding, and, directly mimics protein-DNA interactions observed in the, UDGase-oligonucleotide complex. The inhibitor completely blocks access to, the active site of UDGase, but makes no direct contact with the, uracil-binding pocket itself.
About this Structure
1UDI is a Protein complex structure of sequences from Human herpesvirus 4 and Phage pbs1. Active as Uridine nucleosidase, with EC number 3.2.2.3 Full crystallographic information is available from OCA.
Reference
Nucleotide mimicry in the crystal structure of the uracil-DNA glycosylase-uracil glycosylase inhibitor protein complex., Savva R, Pearl LH, Nat Struct Biol. 1995 Sep;2(9):752-7. PMID:7552746
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