Green Fluorescent Protein
From Proteopedia
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==Structure== | ==Structure== | ||
| - | The crystal structure of GFP <ref>Crystal structure of the Aequorea victoria green fluorescent protein., Ormö M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science 1996 6;273(5280):1392-1395. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703075 8703075]</ref> | + | The crystal structure of GFP <ref>Crystal structure of the Aequorea victoria green fluorescent protein., Ormö M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science 1996 6;273(5280):1392-1395. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703075 8703075]</ref><ref>The molecular structure of green fluorescent protein., Yang F, Moss LG, Phillips GN Jr, Nat Biotechnol. 1996 Oct;14(10):1246-51. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/9631087 9631087]</ref> is an eleven-stranded anti-parallel beta- |
barrel, threaded by an alpha-helix, running up along the axis of the cylinder. | barrel, threaded by an alpha-helix, running up along the axis of the cylinder. | ||
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The chromophore <scene name='Green_Fluorescent_Protein/1ema_chromophorecloseup/2'>close-up</scene> | The chromophore <scene name='Green_Fluorescent_Protein/1ema_chromophorecloseup/2'>close-up</scene> | ||
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| + | ==Reference for the Structure== | ||
| + | Crystal structure of the Aequorea victoria green fluorescent protein., Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science. 1996 Sep 6;273(5280):1392-5. PMID:[http://www.ncbi.nlm.nih.gov/pubmed/8703075 8703075] | ||
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| + | ==Related Structures and Topics== | ||
| + | [[1gfl]] Aequorea victoria Green Fluorescent Protein | ||
| + | [[1b9c]] Aequorea victoria Green Fluorescent Protein Mutant F99s, M153t And V163a | ||
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| + | ==Notes and Literature References== | ||
| + | <references/> | ||
== Additional information on green fluorescent protein == | == Additional information on green fluorescent protein == | ||
Revision as of 03:31, 21 November 2008
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Contents |
Background
Osamu Shimomura, Martin Chalfie and Roger Y. Tsien shared the 2008 Nobel Prize in Chemistry for their for the discovery and development of the green fluorescent protein, GFP.
GFP is small protein (21 kDa) and does not require cofactors to become fluorescent.
Structure
The crystal structure of GFP [1][2] is an eleven-stranded anti-parallel beta- barrel, threaded by an alpha-helix, running up along the axis of the cylinder.
The chromophore is in the distorted alpha-helix that runs along the axis of the can, close to the center of the can-like cylinder.
You can take a close look at the of GFP in the PDB entry 1ema.
The chromophore
Reference for the Structure
Crystal structure of the Aequorea victoria green fluorescent protein., Ormo M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science. 1996 Sep 6;273(5280):1392-5. PMID:8703075
Related Structures and Topics
1gfl Aequorea victoria Green Fluorescent Protein 1b9c Aequorea victoria Green Fluorescent Protein Mutant F99s, M153t And V163a
Notes and Literature References
- ↑ Crystal structure of the Aequorea victoria green fluorescent protein., Ormö M, Cubitt AB, Kallio K, Gross LA, Tsien RY, Remington SJ, Science 1996 6;273(5280):1392-1395. PMID:8703075
- ↑ The molecular structure of green fluorescent protein., Yang F, Moss LG, Phillips GN Jr, Nat Biotechnol. 1996 Oct;14(10):1246-51. PMID:9631087
Additional information on green fluorescent protein
- Roger Y. Tsien (1998) The Green Fluorescent Protein. Annual Review of Biochemistry 67, 509-544.
- [The GFP site] by Marc Zimmer, Ph. D., at Connecticut College, who authored [Glowing Genes: A Revolution In Biotechnology].
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Michal Harel, Wayne Decatur, Karsten Theis, Eran Hodis, Laura Carbone, Karl Oberholser, Mark Hoelzer, Joel L. Sussman, Alexander Berchansky, Jaime Prilusky, Joseph M. Steinberger, David Canner
