1uj0

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spinqualitylabelsall models 1uj0, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal Structure of STAM2 SH3 domain in complex with a UBPY-derived peptide

Overview

Although some exceptional motifs have been identified, it is well known, that the PXXP motif is the motif of ligand proteins generally recognized, by the Src homology 3 (SH3) domain. SH3-ligand interactions are usually, weak, with ordinary KD approximately 10 microM. The structural basis for a, tight and specific association (KD = 0.24 microm) between Gads SH3 and a, novel motif, PX(V/I)(D/N)RXXKP, was revealed in a previous structural, analysis of the complex formed between them. In this paper, we report the, crystal structure of the signal transducing adaptor molecule-2 (STAM2) SH3, domain in complex with a peptide with a novel motif derived from a ligand, protein, UBPY. The derived KD value for this complex is 27 microM. The, notable difference in affinity for these parallel complexes may be, explained because the STAM2 SH3 structure does not provide a specificity, pocket for binding, whereas the Gads SH3 structure does. Instead, the, structure of STAM2 SH3 is analogous to that of Grb2 SH3 which, in addition, to normal PXXP ligands, has also been shown to moderately recognize the, novel motif discussed herein. Thus, the extremely tight interaction, observed between Gads SH3 and the novel motif is caused not by an innate, ability of the novel motif but rather by an evolutionary change in the, Gads SH3 domain. Instead, SH3 domains of STAM2 and Grb2 retain the, moderate characteristics of recognizing their ligand proteins like other, SH3 domains for appropriate transient interactions between signaling, molecules.

About this Structure

1UJ0 is a Protein complex structure of sequences from Mus musculus with PO4 as ligand. Full crystallographic information is available from OCA.

Reference

Structural insight into modest binding of a non-PXXP ligand to the signal transducing adaptor molecule-2 Src homology 3 domain., Kaneko T, Kumasaka T, Ganbe T, Sato T, Miyazawa K, Kitamura N, Tanaka N, J Biol Chem. 2003 Nov 28;278(48):48162-8. Epub 2003 Sep 16. PMID:13129930

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