1ukv
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1ukv" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ukv, resolution 1.50Å" /> '''Structure of RabGDP-...)
Next diff →
Revision as of 02:05, 21 November 2007
|
Structure of RabGDP-dissociation inhibitor in complex with prenylated YPT1 GTPase
Overview
Rab/Ypt guanosine triphosphatases (GTPases) represent a family of key, membrane traffic regulators in eukaryotic cells whose function is governed, by the guanosine diphosphate (GDP) dissociation inhibitor (RabGDI). Using, a combination of chemical synthesis and protein engineering, we generated, and crystallized the monoprenylated Ypt1:RabGDI complex. The structure of, the complex was solved to 1.5 angstrom resolution and provides a, structural basis for the ability of RabGDI to inhibit the release of, nucleotide by Rab proteins. Isoprenoid binding requires a conformational, change that opens a cavity in the hydrophobic core of its domain II., Analysis of the structure provides a molecular basis for understanding a, RabGDI mutant that causes mental retardation in humans.
About this Structure
1UKV is a Protein complex structure of sequences from Saccharomyces cerevisiae with MG, GER and GDP as ligands. Full crystallographic information is available from OCA.
Reference
Structure of Rab GDP-dissociation inhibitor in complex with prenylated YPT1 GTPase., Rak A, Pylypenko O, Durek T, Watzke A, Kushnir S, Brunsveld L, Waldmann H, Goody RS, Alexandrov K, Science. 2003 Oct 24;302(5645):646-50. PMID:14576435
Page seeded by OCA on Wed Nov 21 04:12:24 2007
