1un2
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(New page: 200px<br /><applet load="1un2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1un2, resolution 2.40Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 02:08, 21 November 2007
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CRYSTAL STRUCTURE OF CIRCULARLY PERMUTED CPDSBA_Q100T99 PRESERVED GLOBAL FOLD AND LOCAL STRUCTURAL ADJUSTMENTS
Overview
The thiol-disulfide oxidoreductase DsbA is required for efficient, formation of disulfide bonds in the Escherichia coli periplasm. The enzyme, is the strongest oxidant of the family of thioredoxin-like proteins and, three-dimensional structures of both oxidized and reduced forms are known., DsbA consists of a catalytic thioredoxin-like domain and a helical domain, that is inserted into the thioredoxin motif. Here, the X-ray structure of, a circularly permuted variant, cpDsbA(Q100T99), is reported in which the, natural termini are joined by the pentapeptide linker GGGTG, leading to a, continuous thioredoxin domain, and new termini that have been introduced, in the helical domain by breaking the peptide bond Thr99-Gln100., cpDsbA(Q100T99) is catalytically active in vivo and in vitro. The crystal, structure of oxidized cpDsbA(Q100T99), determined by molecular replacement, at 2.4 A resolution, was found to be very similar to that of wild-type, DsbA. The lower thermodynamic stability of cpDsbA(Q100T99) relative to, DsbA is associated with small structural changes within the molecule, especially near the new termini and the circularizing linker. The, active-site helices and adjacent loops display increased flexibility, compared with oxidized DsbA.
About this Structure
1UN2 is a Single protein structure of sequence from Escherichia coli. This structure superseeds the now removed PDB entry 1DYV. Full crystallographic information is available from OCA.
Reference
Structure of circularly permuted DsbA(Q100T99): preserved global fold and local structural adjustments., Manjasetty BA, Hennecke J, Glockshuber R, Heinemann U, Acta Crystallogr D Biol Crystallogr. 2004 Feb;60(Pt 2):304-9. Epub 2004, Jan 23. PMID:14747707
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