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1uty
From Proteopedia
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(New page: 200px<br /><applet load="1uty" size="450" color="white" frame="true" align="right" spinBox="true" caption="1uty, resolution 2.40Å" /> '''CRYSTAL STRUCTURE OF...)
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Revision as of 02:12, 21 November 2007
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CRYSTAL STRUCTURE OF THE RNA BINDING DOMAIN OF BLUETONGUE VIRUS NON-STRUCTURAL PROTEIN 2(NS2)
Overview
Bluetongue virus non-structural protein 2 belongs to a class of highly, conserved proteins found in orbiviruses of the Reoviridae family., Non-structural protein 2 forms large multimeric complexes and localizes to, cytoplasmic inclusions in infected cells. It is able to bind, single-stranded RNA non-specifically, and it has been suggested that the, protein is involved in the selection and condensation of the Bluetongue, virus RNA segments prior to genome encapsidation. We have determined the, x-ray structure of the N-terminal domain (sufficient for the RNA binding, ability of non-structural protein 2) to 2.4 A resolution using anomalous, scattering methods. Crystals of this apparently insoluble domain were, obtained by in situ proteolysis of a soluble construct. The asymmetric, unit shows two monomers related by non-crystallographic symmetry, with, each monomer folded as a beta sandwich with a unique topology. The crystal, structure reveals extensive monomer-monomer interactions, which explain, the ability of the protein to self-assemble into large homomultimeric, complexes. Of the entire surface area of the monomer, one-third is used to, create the interfaces of the curved multimeric assembly observed in the, x-ray structure. The structure reported here shows how the N-terminal, domain would be able to bind single-stranded RNA non-specifically, protecting the bound regions in a heterogeneous multimeric but not, polymeric complex.
About this Structure
1UTY is a Single protein structure of sequence from Bluetongue virus. Active as RNA-directed RNA polymerase, with EC number 2.7.7.48 Full crystallographic information is available from OCA.
Reference
Structure and assembly of the RNA binding domain of bluetongue virus non-structural protein 2., Butan C, Van Der Zandt H, Tucker PA, J Biol Chem. 2004 Sep 3;279(36):37613-21. Epub 2004 May 20. PMID:15155766
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