User:Kay Owosela
From Proteopedia
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| - | ( | + | In this enzyme, there are 12 similar subunits bonded to each other. Each subunit has an active site. The active site of glutamine synthetase lies primarily around 3 manganese atoms. It is surrounded by 3 important ligands: MPD, ADP, TL as labeled in the applet. ADP is short for Adenosite Diphosphate. The ligand MPD is (4s)-2-methyl-2,4-pentanediol. And the metal ion, Thallium is also labeled in the applet. |
<scene name='User:Kay_Owosela/Active_site/3'>Active Site</scene> | <scene name='User:Kay_Owosela/Active_site/3'>Active Site</scene> | ||
= Glutamine Synthetase Symmetry = | = Glutamine Synthetase Symmetry = | ||
<scene name='User:Kay_Owosela/Sandwich_symmetry/1'>Sandwich symmetry</scene> | <scene name='User:Kay_Owosela/Sandwich_symmetry/1'>Sandwich symmetry</scene> | ||
Revision as of 03:50, 8 December 2008
Biol 430 Biology Major Partners: Delo Sarr, Mustapha Hussain
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Contents |
Jmol practice
Backbone with ligand
Labeled Ligands in Chain A
Active Site with Amino Acid Residues
Connection between Chain F residue 63 and Chain G residue 319
Stoichiometry
The stoichiometry of Glutamine Synthetase is as follows: Glutamate + NHз + ATP --> Glutamine + ADP + Pi + H+
In this reaction, Glutamate is used as a major reactant and ATP is used for energy to form Glutamine. It should be noted that along with the enzyme, Me+ which can be either manganese or magnesium is used as a catalyst.
This is however, a 2-step reaction. In the first step of this reaction, an intermediate Υ-glutamyl phosphate is formed. The formation of the intermediate is done by phosphoryl transfer an ATP group Y-carboxylate group of glutamate. In the second step of this reaction, NHз attacks the activated intermediate to release the phosphate group yielding Glutamine.
Quarternary Structure Polymerization
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Active Site
In this enzyme, there are 12 similar subunits bonded to each other. Each subunit has an active site. The active site of glutamine synthetase lies primarily around 3 manganese atoms. It is surrounded by 3 important ligands: MPD, ADP, TL as labeled in the applet. ADP is short for Adenosite Diphosphate. The ligand MPD is (4s)-2-methyl-2,4-pentanediol. And the metal ion, Thallium is also labeled in the applet.
Glutamine Synthetase Symmetry
