User:Steven Tuyishime
From Proteopedia
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<scene name='User:Steven_Tuyishime/Domain_from_1_to_103/1'> | <scene name='User:Steven_Tuyishime/Domain_from_1_to_103/1'> | ||
| - | The second domain in S. typhimurium begins at residue 1 and goes to residue 103</scene>. In this domain, the architecture is a roll. The topology is <scene name='User:Steven_Tuyishime/Ubiquitin_like_roll/2'>ubiquitin-like, or a UB-roll</scene> and the Homologous superfamily has no defined name. This domain is smaller and has an NH2 terminus. <scene name='User:Steven_Tuyishime/Covalent_linkage_103_to_104/ | + | The second domain in S. typhimurium begins at residue 1 and goes to residue 103</scene>. In this domain, the architecture is a roll. The topology is <scene name='User:Steven_Tuyishime/Ubiquitin_like_roll/2'>ubiquitin-like, or a UB-roll</scene> and the Homologous superfamily has no defined name. This domain is smaller and has an NH2 terminus. |
| + | <scene name='User:Steven_Tuyishime/Covalent_linkage_103_to_104/3'>It is covalently linked to the larger COOH terminus</scene> (the first domain described in prior paragraph). This NH2 terminus is exposed compared to the buried COOH terminus of the larger domain <ref> Yamashita MM, Almassy RJ, Janson CA, Cascio D, EisenberDg . 1989. JBiol Chem 264:17681-17690. Refined atomic model of glutamine synthetase at 3.5 A resolution.</ref>. | ||
Revision as of 00:47, 19 December 2008
Undergraduate student at UMBC, Group consisits of Sindhu Lakkur, Ejiofor Ezekwe and Steven Tuyishime- Dr. Thomas Gluick Biol 430
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Glutamine Synthetase CATH Domains
Glutamine Synthetase (GS) has 2 prominent CATH domains. CATH is an acronym for Class (C), Architecture (A), Topology (T), and Homologous superfamily (H). Each of these is a way to classify molecules. A molecule can be classified by any one of 4 classes: Mainly Alpha, Mainly Beta, Alpha Beta, and Few Secondary Structures [1]. Another level of classification which is on a lower order than class is Architecture. This level of classification is used to identify the shape of the domain structure using the orientation of secondary structures but ignoring the way they are connected to each other [1]. A third level of classification is Topology, which is also called Fold Group. This classification concerns the shared similarity of overall shape and connectivity of the secondary structures in the domain core [1]. The Homologous superfamily refers to a fourth classification type. This hierarchy groups together protein domains which are thought to share a common ancestor, according to specific criteria [1].
Looking at Glutamine Synthetase in Salmonella typhimurium, we can identify 2 CATH domains. Each of the domains are of the class Alpha Beta, however, that is the only common aspect between the two. Alpha Beta includes both alternating alpha/beta structures and alpha+beta structures. . For this domain, the Architecture is a . The Topology is Creatine Kinase; Chain A, domain 2 and the Homologous superfamily is Creatine Kinase; Chain. This domain is the larger of the two and has a COOH terminus.
. In this domain, the architecture is a roll. The topology is and the Homologous superfamily has no defined name. This domain is smaller and has an NH2 terminus. (the first domain described in prior paragraph). This NH2 terminus is exposed compared to the buried COOH terminus of the larger domain [2].
