1waf
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(New page: 200px<br /><applet load="1waf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1waf, resolution 3.2Å" /> '''DNA POLYMERASE FROM B...)
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Revision as of 03:09, 21 November 2007
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DNA POLYMERASE FROM BACTERIOPHAGE RB69
Overview
The 2.8 A resolution crystal structure of the bacteriophage RB69 gp43, a, member of the eukaryotic pol alpha family of replicative DNA polymerases, shares some similarities with other polymerases but shows many, differences. Although its palm domain has the same topology as other, polymerases, except rat DNA polymerase beta, one of the three carboxylates, required for nucleotidyl transfer is located on a different beta strand., The structures of the fingers and thumb domains are unrelated to all other, known polymerase structures. The editing 3'-5' exonuclease domain of gp43, is homologous to that of E. coli DNA polymerase I but lies on the opposite, side of the polymerase active site. An extended structure-based alignment, of eukaryotic DNA polymerase sequences provides structural insights that, should be applicable to most eukaryotic DNA polymerases.
About this Structure
1WAF is a Single protein structure of sequence from Enterobacteria phage rb18 with GMP as ligand. Active as DNA-directed DNA polymerase, with EC number 2.7.7.7 Full crystallographic information is available from OCA.
Reference
Crystal structure of a pol alpha family replication DNA polymerase from bacteriophage RB69., Wang J, Sattar AK, Wang CC, Karam JD, Konigsberg WH, Steitz TA, Cell. 1997 Jun 27;89(7):1087-99. PMID:9215631
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