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1wij
From Proteopedia
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(New page: 200px<br /><applet load="1wij" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wij" /> '''Solution Structure of the DNA-Binding Domain...)
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Revision as of 03:20, 21 November 2007
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Solution Structure of the DNA-Binding Domain of Ethylene-Insensitive3-Like3
Overview
Ethylene-insensitive3 (EIN3) and EIN3-like (EIL) proteins are essential, transcription factors in the ethylene signaling of higher plants. The, EIN3/EIL proteins bind to the promoter regions of the downstream genes and, regulate their expression. The location of the DNA-binding domain (DBD) in, the primary structure was unclear, since the proteins show no sequence, similarity to other known DBDs. Here, we identify the major DBD of an, EIN3/EIL protein, Arabidopsis thaliana EIL3, containing a key mutational, site for DNA binding and signaling (ein3-3 site), and determine its, solution structure by NMR spectroscopy. The structure consists of five, alpha-helices, possessing a novel fold dissimilar to known DBD structures., By a chemical-shift perturbation analysis, a region including the ein3-3, site is suggested to be involved in DNA binding.
About this Structure
1WIJ is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.
Reference
Solution structure of the major DNA-binding domain of Arabidopsis thaliana ethylene-insensitive3-like3., Yamasaki K, Kigawa T, Inoue M, Yamasaki T, Yabuki T, Aoki M, Seki E, Matsuda T, Tomo Y, Terada T, Shirouzu M, Tanaka A, Seki M, Shinozaki K, Yokoyama S, J Mol Biol. 2005 Apr 29;348(2):253-64. PMID:15811366
Page seeded by OCA on Wed Nov 21 05:27:18 2007
