This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1wm2
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1wm2" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wm2, resolution 1.6Å" /> '''Crystal structure of ...)
Next diff →
Revision as of 03:24, 21 November 2007
|
Crystal structure of human SUMO-2 protein
Overview
The SUMO proteins are a class of small ubiquitin-like modifiers. SUMO is, attached to a specific lysine side chain on the target protein via an, isopeptide bond with its C-terminal glycine. There are at least four SUMO, proteins in humans, which are involved in protein trafficking and, targeting. A truncated human SUMO-2 protein that contains residues 9-93, was expressed in Escherichia coli and crystallized in two different unit, cells, with dimensions of a=b=75.25 A, c=29.17 A and a=b=74.96 A, c=33.23, A, both belonging to the rhombohedral space group R3. They diffracted, X-rays to 1.6 A and 1.2 A resolution, respectively. The structures were, determined by molecular replacement using the yeast SMT3 protein as a, search model. Subsequent refinements yielded R/Rfree values of 0.169/0.190, and 0.119/0.185, at 1.6 A and 1.2 A, respectively. The peptide folding of, SUMO-2 consists of a half-open beta-barrel and two flanking alpha-helices, with secondary structural elements arranged as, betabetaalphabetabetaalphabeta in the sequence, identical to those of, ubiquitin, SMT3 and SUMO-1. Comparison of SUMO-2 with SUMO-1 showed a, surface region near the C terminus with significantly different charge, distributions. This may explain their distinct intracellular locations. In, addition, crystal-packing analysis suggests a possible trimeric assembly, of the SUMO-2 protein, of which the biological significance remains to be, determined.
About this Structure
1WM2 is a Single protein structure of sequence from Homo sapiens. Full crystallographic information is available from OCA.
Reference
Crystal structures of the human SUMO-2 protein at 1.6 A and 1.2 A resolution: implication on the functional differences of SUMO proteins., Huang WC, Ko TP, Li SS, Wang AH, Eur J Biochem. 2004 Oct;271(20):4114-22. PMID:15479240
Page seeded by OCA on Wed Nov 21 05:31:21 2007
