1wwl

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Revision as of 03:36, 21 November 2007

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spinqualitylabelsall models 1wwl, resolution 2.50Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of CD14

Overview

Lipopolysaccharide, the endotoxin of Gram-negative bacteria, induces, extensive immune responses that can lead to fatal septic shock syndrome., The core receptors recognizing lipopolysaccharide are CD14, TLR4, and, MD-2. CD14 binds to lipopolysaccharide and presents it to the TLR4/MD-2, complex, which initiates intracellular signaling. In addition to, lipopolysaccharide, CD14 is capable of recognizing a few other microbial, and cellular products. Here, we present the first crystal structure of, CD14 to 2.5 angstroms resolution. A large hydrophobic pocket was found on, the NH2-terminal side of the horseshoe-like structure. Previously, identified regions involved in lipopolysaccharide binding map to the rim, and bottom of the pocket indicating that the pocket is the main component, of the lipopolysaccharide-binding site. Mutations that interfere with, lipopolysaccharide signaling but not with lipopolysaccharide binding are, also clustered in a separate area near the pocket. Ligand diversity of, CD14 could be explained by the generous size of the pocket, the, considerable flexibility of the rim of the pocket, and the multiplicity of, grooves available for ligand binding.

About this Structure

1WWL is a Single protein structure of sequence from Mus musculus with NAG as ligand. Full crystallographic information is available from OCA.

Reference

Crystal structure of CD14 and its implications for lipopolysaccharide signaling., Kim JI, Lee CJ, Jin MS, Lee CH, Paik SG, Lee H, Lee JO, J Biol Chem. 2005 Mar 25;280(12):11347-51. Epub 2005 Jan 10. PMID:15644310

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