1wzz

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(New page: 200px<br /><applet load="1wzz" size="450" color="white" frame="true" align="right" spinBox="true" caption="1wzz, resolution 1.65&Aring;" /> '''Structure of endo-be...)
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Revision as of 03:38, 21 November 2007

Image:1wzz.gif

1wzz, resolution 1.65Å

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Structure of endo-beta-1,4-glucanase CMCax from Acetobacter xylinum

Overview

Previous studies have demonstrated that endoglucanase is required for, cellulose biosynthesis both in bacteria and plants. However, it has yet to, be elucidated how the endoglucanases function in the mechanism of, cellulose biosynthesis. Here we describe the crystal structure of the, cellulose biosynthesis-related endo-beta-1,47-glucanase (CMCax; EC, 3.2.1.4) from the cellulose-producing Gramnegative bacterium, Acetobacter, xylinum (= Gluconacetobacter xylinus), determined at 1.65-A resolution., CMCax falls into the glycoside hydrolase family 8 (GH-8), and the, structure showed that the overall fold of the CMCax is similar to those of, other glycoside hydrolases belonging to GH-8. Structure comparison with, Clostridium thermocellum CelA, the best characterized GH-8 endoglucanase, revealed that sugar recognition subsite +3 is completely missing in CMCax., The absence of the subsite +3 leads to significant broadness of the cleft, at the cellooligosaccharide reducing-end side. CMCax is known to be a, secreted enzyme and is present in the culture medium. However, electron, microscopic analysis using immunostaining clearly demonstrated that a, portion of CMCax is localized to the cell surface, suggesting a link with, other known membrane-anchored endoglucanases that are required for, cellulose biosynthesis.

About this Structure

1WZZ is a Single protein structure of sequence from Gluconacetobacter xylinus with SO4 as ligand. Active as Cellulase, with EC number 3.2.1.4 Full crystallographic information is available from OCA.

Reference

Structural characterization of the Acetobacter xylinum endo-beta-1,4-glucanase CMCax required for cellulose biosynthesis., Yasutake Y, Kawano S, Tajima K, Yao M, Satoh Y, Munekata M, Tanaka I, Proteins. 2006 Sep 1;64(4):1069-77. PMID:16804941

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