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2ek8
From Proteopedia
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{{STRUCTURE_2ek8| PDB=2ek8 | SCENE= }} | {{STRUCTURE_2ek8| PDB=2ek8 | SCENE= }} | ||
| - | + | ===Aminopeptidase from Aneurinibacillus sp. strain AM-1=== | |
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| - | ==Overview== | ||
| - | To elucidate the structure and molecular mechanism of a characteristic proline-specific aminopeptidase produced by the thermophile Aneurinibacillus sp. strain AM-1, its gene was cloned and the recombinant protein was overexpressed in Escherichia coli, purified and crystallized using the hanging-drop vapour-diffusion method. X-ray diffraction data were collected to 1.8 A resolution from the recombinant aminopeptidase crystal. The crystals belong to the orthorhombic space group P2(1)2(1)2, with unit-cell parameters a = 93.62, b = 68.20, c = 76.84 A. A complete data set was also obtained from crystals of SeMet-substituted aminopeptidase. Data in the resolution range 20-2.1 A from the MAD data set from the SeMet-substituted crystal were used for phase determination. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2EK8 is a | + | 2EK8 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bacteria Bacteria]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2EK8 OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:17142913</ref><references group="xtra"/> | |
[[Category: Bacteria]] | [[Category: Bacteria]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Akioka, M.]] | [[Category: Akioka, M.]] | ||
[[Category: Nakano, H.]] | [[Category: Nakano, H.]] | ||
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[[Category: Hydrolase]] | [[Category: Hydrolase]] | ||
[[Category: Metalloproteinase]] | [[Category: Metalloproteinase]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 11:43:26 2009'' | ||
Revision as of 09:43, 16 February 2009
Aminopeptidase from Aneurinibacillus sp. strain AM-1
About this Structure
2EK8 is a 1 chain structure of sequence from Bacteria. Full crystallographic information is available from OCA.
Reference
- Akioka M, Nakano H, Horikiri A, Tsujimoto Y, Matsui H, Shimizu T, Nakatsu T, Kato H, Watanabe K. Overexpression, purification, crystallization and preliminary X-ray crystallographic studies of a proline-specific aminopeptidase from Aneurinibacillus sp. strain AM-1. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2006 Dec 1;62(Pt, 12):1266-8. Epub 2006 Nov 30. PMID:17142913 doi:10.1107/S1744309106047543
Page seeded by OCA on Mon Feb 16 11:43:26 2009
