1xfl

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Solution Structure of Thioredoxin h1 from Arabidopsis Thaliana

Overview

Present in virtually every species, thioredoxins catalyze, disulfide/dithiol exchange with various substrate proteins. While the, human genome contains a single thioredoxin gene, plant thioredoxins are a, complex protein family. A total of 19 different thioredoxin genes in six, subfamilies has emerged from analysis of the Arabidopsis thaliana genome., Some function specifically in mitochondrial and chloroplast redox, signaling processes, but target substrates for a group of eight, thioredoxin proteins comprising the h subfamily are largely, uncharacterized. In the course of a structural genomics effort directed at, the recently completed A. thaliana genome, we determined the structure of, thioredoxin h1 (At3g51030.1) in the oxidized state. The structure, defined, by 1637 NMR-derived distance and torsion angle constraints, displays the, conserved thioredoxin fold, consisting of a five-stranded beta-sheet, flanked by four helices. Redox-dependent chemical shift perturbations, mapped primarily to the conserved WCGPC active-site sequence and other, nearby residues, but distant regions of the C-terminal helix were also, affected by reduction of the active-site disulfide. Comparisons of the, oxidized A. thaliana thioredoxin h1 structure with an h-type thioredoxin, from poplar in the reduced state revealed structural differences in the, C-terminal helix but no major changes in the active site conformation.

About this Structure

1XFL is a Single protein structure of sequence from Arabidopsis thaliana. Full crystallographic information is available from OCA.

Reference

Solution structure of thioredoxin h1 from Arabidopsis thaliana., Peterson FC, Lytle BL, Sampath S, Vinarov D, Tyler E, Shahan M, Markley JL, Volkman BF, Protein Sci. 2005 Aug;14(8):2195-200. Epub 2005 Jun 29. PMID:15987893

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