1xkg
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(New page: 200px<br /><applet load="1xkg" size="450" color="white" frame="true" align="right" spinBox="true" caption="1xkg, resolution 1.61Å" /> '''Crystal structure of...)
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Revision as of 04:00, 21 November 2007
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Crystal structure of the major house dust mite allergen Der p 1 in its pro form at 1.61 A resolution
Overview
Allergy to house dust mite is among the most prevalent allergic diseases, worldwide. Most house dust mite allergic patients react to Der p 1 from, Dermatophagoides pteronyssinus, which is a cysteine protease. To avoid, heterogeneity in the sample used for crystallization, a modified, recombinant molecule was produced. The sequence of the proDer p 1 allergen, was modified to reduce glycosylation and to abolish enzymatic activity., The resulting rproDer p 1 preparation was homogenous and stable and, yielded crystals diffracting to a resolution of 1.61 A. The active site is, located in a large cleft on the surface of the molecule. The 80-aa, pro-peptide adopts a unique fold that interacts with the active site cleft, and a substantial adjacent area on the mature region, excluding access to, the cleft and the active site. Studies performed using crossed-line, immunoelectrophoresis and IgE inhibition experiments indicated that, several epitopes are covered by the pro-peptide and that the epitopes on, the recombinant mature molecule are indistinguishable from those on the, natural one. The structure confirms previous results suggesting a, preference for aliphatic residues in the important P2 position in, substrates. Sequence variations in related species are concentrated on the, surface, which explains the existence of cross-reacting and, species-specific antibodies. This study describes the first crystal, structure of one of the clinically most important house dust mite, allergens, the cysteine protease Der p 1.
About this Structure
1XKG is a Single protein structure of sequence from Dermatophagoides pteronyssinus with YT3, SO4 and GOL as ligands. Full crystallographic information is available from OCA.
Reference
The crystal structure of recombinant proDer p 1, a major house dust mite proteolytic allergen., Meno K, Thorsted PB, Ipsen H, Kristensen O, Larsen JN, Spangfort MD, Gajhede M, Lund K, J Immunol. 2005 Sep 15;175(6):3835-45. PMID:16148130
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