1xku

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spinqualitylabelsall models 1xku, resolution 2.15Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the dimeric protein core of decorin, the archetypal small leucine-rich repeat proteoglycan

Overview

Decorin is a ubiquitous extracellular matrix proteoglycan with a variety, of important biological functions that are mediated by its interactions, with extracellular matrix proteins, cytokines, and cell surface receptors., Decorin is the prototype of the family of small leucine-rich repeat, proteoglycans and proteins (SLRPs), characterized by a protein core, composed of leucine-rich repeats (LRRs), flanked by two cysteine-rich, regions. We report here the crystal structure of the dimeric protein core, of decorin, the best characterized member of the SLRP family. Each monomer, adopts the curved solenoid fold characteristic of LRR domains, with a, parallel beta-sheet on the inside interwoven with loops containing short, segments of beta-strands, 3(10) helices, and polyproline II helices on the, outside. Two main features are unique to this structure. First, decorin, dimerizes through the concave surfaces of the LRR domains, which have been, implicated previously in protein-ligand interactions. The amount of, surface buried in this dimer rivals the buried surfaces of some of the, highest-affinity macromolecular complexes reported to date. Second, the, C-terminal region adopts an unusual capping motif that involves a, laterally extended LRR and a disulfide bond. This motif seems to be unique, to SLRPs and has not been observed in any other LRR protein structure to, date. Possible implications of these features for decorin ligand binding, and SLRP function are discussed.

About this Structure

1XKU is a Single protein structure of sequence from Bos taurus with NAG and TRS as ligands. Full crystallographic information is available from OCA.

Reference

Crystal structure of the dimeric protein core of decorin, the archetypal small leucine-rich repeat proteoglycan., Scott PG, McEwan PA, Dodd CM, Bergmann EM, Bishop PN, Bella J, Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15633-8. Epub 2004 Oct 22. PMID:15501918

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