3d91
From Proteopedia
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{{STRUCTURE_3d91| PDB=3d91 | SCENE= }} | {{STRUCTURE_3d91| PDB=3d91 | SCENE= }} | ||
| - | + | ===Human renin in complex with remikiren=== | |
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| - | ==Overview== | ||
| - | Human renin has been expressed in Sf9 and CHO cells using two different gene constructs. The first construct contained a foreign signal peptide fused directly to the sequence encoding mature renin, whereas the second construct harbors the sequence for preprorenin. Prorenin was produced in significantly higher amounts than the mature enzyme expressed without its propeptide in both expression systems. Both directly expressed mature renin and proteolytically derived active renin have been purified and cocrystallized with the renin inhibitor Ro 42-5892. The 3D structure has been solved for both versions and demonstrates identity despite different glycosylation and different N termini. | ||
==Disease== | ==Disease== | ||
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==About this Structure== | ==About this Structure== | ||
| - | 3D91 is a | + | 3D91 is a 2 chains structure of sequences from [http://en.wikipedia.org/wiki/Homo_sapiens Homo sapiens]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=3D91 OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:9172787</ref><references group="xtra"/> | |
[[Category: Homo sapiens]] | [[Category: Homo sapiens]] | ||
[[Category: Renin]] | [[Category: Renin]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Bezencon, O.]] | [[Category: Bezencon, O.]] | ||
[[Category: Bur, D.]] | [[Category: Bur, D.]] | ||
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[[Category: Secreted]] | [[Category: Secreted]] | ||
[[Category: Zymogen]] | [[Category: Zymogen]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 14:31:39 2009'' | ||
Revision as of 12:31, 16 February 2009
Contents |
Human renin in complex with remikiren
Disease
Known disease associated with this structure: Renal tubular dysgenesis OMIM:[179820], Hyperproreninemia OMIM:[179820]
About this Structure
3D91 is a 2 chains structure of sequences from Homo sapiens. Full crystallographic information is available from OCA.
Reference
- Mathews S, Dobeli H, Pruschy M, Bosser R, D'Arcy A, Oefner C, Zulauf M, Gentz R, Breu V, Matile H, Schlaeger J, Fischli W. Recombinant human renin produced in different expression systems: biochemical properties and 3D structure. Protein Expr Purif. 1996 Feb;7(1):81-91. PMID:9172787 doi:10.1006/prep.1996.0012
Page seeded by OCA on Mon Feb 16 14:31:39 2009
Categories: Homo sapiens | Renin | Bezencon, O. | Bur, D. | Fischli, W. | Prade, L. | Remen, L. | Weller, T. | Alternative splicing | Aspartyl protease | Cleavage on pair of basic residue | Disease mutation | Glycoprotein | Hydrolase | Membrane | Polymorphism | Protease | Remikiren | Secreted | Zymogen
