1y4z

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Revision as of 04:26, 21 November 2007

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spinqualitylabelsall models 1y4z, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

The crystal structure of Nitrate Reductase A, NarGHI, in complex with the Q-site inhibitor pentachlorophenol

Overview

The crystal structure of Escherichia coli nitrate reductase A (NarGHI) in, complex with pentachlorophenol has been determined to 2.0 A of resolution., We have shown that pentachlorophenol is a potent inhibitor of, quinol:nitrate oxidoreductase activity and that it also perturbs the EPR, spectrum of one of the hemes located in the membrane anchoring subunit, (NarI). This new structural information together with site-directed, mutagenesis data, biochemical analyses, and molecular modeling provide the, first molecular characterization of a quinol binding and oxidation site, (Q-site) in NarGHI. A possible proton conduction pathway linked to, electron transfer reactions has also been defined, providing fundamental, atomic details of ubiquinol oxidation by NarGHI at the bacterial membrane.

About this Structure

1Y4Z is a Protein complex structure of sequences from Escherichia coli with MD1, 6MO, HEM, SF4, F3S, PCI and AGA as ligands. Active as Nitrate reductase, with EC number 1.7.99.4 Full crystallographic information is available from OCA.

Reference

Structural and biochemical characterization of a quinol binding site of Escherichia coli nitrate reductase A., Bertero MG, Rothery RA, Boroumand N, Palak M, Blasco F, Ginet N, Weiner JH, Strynadka NC, J Biol Chem. 2005 Apr 15;280(15):14836-43. Epub 2004 Dec 22. PMID:15615728

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