1ygt
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(New page: 200px<br /><applet load="1ygt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ygt, resolution 1.7Å" /> '''Dynein Light Chain Tc...)
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Revision as of 04:38, 21 November 2007
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Dynein Light Chain TcTex-1
Overview
TcTex-1, one of three dynein light chains of the dynein motor complex, has, been implicated in targeting and binding cargoes to cytoplasmic dynein for, retrograde or apical transport. Interactions between TcTex-1 and a diverse, set of proteins such as the dynein intermediate chain, Fyn, DOC2, FIP1, the poliovirus receptor, CD155, and the rhodopsin cytoplasmic tail have, been reported; yet, despite the broad range of targets, a consensus, binding sequence remains uncertain. Consequently, we have solved the, crystal structure of the full-length Drosophila homolog of TcTex-1 to 1.7, A resolution using MAD phasing to gain insight into its function and, target specificity. The structure is homodimeric with a domain swapping of, beta-strand 2 and has a fold similar to the dynein light chain, LC8. Based, on structural alignment, the TcTex-1 and LC8 sequences show no identity, although the root mean square deviation between secondary structural, elements is less than 1.6 A. Moreover, the N terminus, which is equivalent, to beta-strand 1 in LC8, is splayed out and binds to a crystallographic, dimer as an anti-parallel beta-strand at the same position as the neuronal, nitric-oxide synthase peptide in the LC8 complex. Similarity to LC8 and, comparison to the LC8-neuronal nitricoxide synthase complex suggest that, TcTex-1 binds its targets in a similar manner as LC8 and provides insight, to the lack of strict sequence identity among the targets for TcTex-1.
About this Structure
1YGT is a Single protein structure of sequence from Drosophila melanogaster with SO4 as ligand. Full crystallographic information is available from OCA.
Reference
Crystal structure of dynein light chain TcTex-1., Williams JC, Xie H, Hendrickson WA, J Biol Chem. 2005 Jun 10;280(23):21981-6. Epub 2005 Feb 8. PMID:15701632
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