1bk9

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Revision as of 19:29, 29 October 2007

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spinqualitylabelsall models 1bk9, resolution 2.00Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

PHOSPHOLIPASE A2 MODIFIED BY PBPB

Overview

The crystal structure of acidic phospholipase A2 (APLA2) from Agkistrodon, halys pallas covalently modified by p-bromo-phenacyl-bromide (pBPB) was, determined to a resolution of 2.0 A by an isomorphous difference Fourier, method with the native APLA2 structure as an initial model and refined to, a crystallographic R factor of 15.3%. The modified APLA2 structure is, remarkably similar to that of the native one. Least-squares superposition, of C alpha atoms of native and modified APLA2 results in a, root-mean-square coordinate deviation of 0.243 A. The p-bromo-phenacyl, group near the active site occupies a position similar to that in pBPB, modified bovine pancreatic PLA2. The inhibitor covalently bound to the NDI, atom of His48 fits well in the hydrophobic channel, forming extensive, ... [(full description)]

About this Structure

1BK9 is a [Single protein] structure of sequence from [Gloydius halys] with CA, PBP and BU1 as [ligands]. Active as [[1]], with EC number [3.1.1.4]. Full crystallographic information is available from [OCA].

Reference

Structure of a snake venom phospholipase A2 modified by p-bromo-phenacyl-bromide., Zhao H, Tang L, Wang X, Zhou Y, Lin Z, Toxicon. 1998 Jun;36(6):875-86. PMID:9663694

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