1ymf

From Proteopedia

Revision as of 04:47, 21 November 2007

crystal structure of yellow fever virus NS3 helicase complexed with ADP

Overview

Yellow fever virus (YFV), a member of the Flavivirus genus, has a, plus-sense RNA genome encoding a single polyprotein. Viral protein NS3, includes a protease and a helicase that are essential to virus replication, and to RNA capping. The 1.8-A crystal structure of the helicase region of, the YFV NS3 protein includes residues 187 to 623. Two familiar helicase, domains bind nucleotide in a triphosphate pocket without base recognition, providing a site for nonspecific hydrolysis of nucleoside triphosphates, and RNA triphosphate. The third, C-terminal domain has a unique structure, and is proposed to function in RNA and protein recognition. The, organization of the three domains indicates that cleavage of the viral, polyprotein NS3-NS4A junction occurs in trans.

About this Structure

1YMF is a Single protein structure of sequence from Yellow fever virus with ADP as ligand. Full crystallographic information is available from OCA.

Reference

Structure of the Flavivirus helicase: implications for catalytic activity, protein interactions, and proteolytic processing., Wu J, Bera AK, Kuhn RJ, Smith JL, J Virol. 2005 Aug;79(16):10268-77. PMID:16051820

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