1ynr
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(New page: 200px<br /><applet load="1ynr" size="450" color="white" frame="true" align="right" spinBox="true" caption="1ynr, resolution 2.00Å" /> '''Crystal structure of...)
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Revision as of 04:48, 21 November 2007
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Crystal structure of the cytochrome c-552 from Hydrogenobacter thermophilus at 2.0 resolution
Overview
The folding mechanism of many proteins involves the population of, partially organized structures en route to the native state., Identification and characterization of these intermediates is particularly, difficult, as they are often only transiently populated and may play, different mechanistic roles, being either on-pathway productive species or, off-pathway kinetic traps. Following different spectroscopic probes, and, employing state-of-the-art kinetic analysis, we present evidence that the, folding mechanism of the thermostable cytochrome c552 from Hydrogenobacter, thermophilus does involve the presence of an elusive, yet compact, on-pathway intermediate. Characterization of the folding mechanism of this, cytochrome c is particularly interesting for the purpose of comparative, folding studies, because H. thermophilus cytochrome c552 shares high, sequence identity and structural homology with its homologue from the, mesophilic bacterium Pseudomonas aeruginosa cytochrome c551, which refolds, through a broad energy barrier without the accumulation of intermediates., Analysis of the folding kinetics and correlation with the, three-dimensional structure add new evidence for the validity of a, consensus folding mechanism in the cytochrome c family.
About this Structure
1YNR is a Single protein structure of sequence from Hydrogenobacter thermophilus with SO4, HEC and MPD as ligands. Full crystallographic information is available from OCA.
Reference
An obligatory intermediate in the folding pathway of cytochrome c552 from Hydrogenobacter thermophilus., Travaglini-Allocatelli C, Gianni S, Dubey VK, Borgia A, Di Matteo A, Bonivento D, Cutruzzola F, Bren KL, Brunori M, J Biol Chem. 2005 Jul 8;280(27):25729-34. Epub 2005 May 9. PMID:15883159
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