1yoe
From Proteopedia
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{{STRUCTURE_1yoe| PDB=1yoe | SCENE= }} | {{STRUCTURE_1yoe| PDB=1yoe | SCENE= }} | ||
| - | + | ===Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose=== | |
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| - | ==Overview== | ||
| - | Enzymes with nucleoside hydrolase (NH) activity are crucial for salvaging nucleic acid components in purine auxotrophic protozoan parasites, but are also present in prokaryotes and higher eukaryotes. Here we analyze the distribution of genes encoding for putative NH proteins and characterize the yeiK gene product from Escherichia coli as a pyrimidine-specific NH. The crystal structure of YeiK to 1.7 A defines the structural basis for its substrate specificity and identifies residues involved in the catalytic mechanism that differ from both nonspecific and purine-specific NHs. Large differences in the tetrameric quaternary structure compared to nonspecific protozoan NHs are brought forth by minor differences in the interacting surfaces. The first structural and functional characterization of a nonparasitic, pyrimidine nucleoside-specific NH suggests a possible role for these enzymes in the metabolism of tRNA nucleosides. The high catalytic efficiency of YeiK toward 5-fluorouridine could be exploited for suicide gene therapy in cancer treatment. | ||
==About this Structure== | ==About this Structure== | ||
| - | 1YOE is a | + | 1YOE is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Escherichia_coli Escherichia coli]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=1YOE OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:15130467</ref><ref group="xtra">PMID:11292348</ref><ref group="xtra">PMID:9572842</ref><references group="xtra"/> | |
[[Category: Escherichia coli]] | [[Category: Escherichia coli]] | ||
[[Category: Ribosylpyrimidine nucleosidase]] | [[Category: Ribosylpyrimidine nucleosidase]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Degano, M.]] | [[Category: Degano, M.]] | ||
[[Category: Muzzolini, L.]] | [[Category: Muzzolini, L.]] | ||
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[[Category: Pyrimidine nucleoside hydrolase]] | [[Category: Pyrimidine nucleoside hydrolase]] | ||
[[Category: Ribose]] | [[Category: Ribose]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 17:52:23 2009'' | ||
Revision as of 15:52, 16 February 2009
Crystal structure of a the E. coli pyrimidine nucleoside hydrolase YbeK with bound ribose
About this Structure
1YOE is a 1 chain structure of sequence from Escherichia coli. Full crystallographic information is available from OCA.
Reference
- Giabbai B, Degano M. Crystal structure to 1.7 a of the Escherichia coli pyrimidine nucleoside hydrolase YeiK, a novel candidate for cancer gene therapy. Structure. 2004 May;12(5):739-49. PMID:15130467 doi:http://dx.doi.org/10.1016/j.str.2004.03.018
- Versees W, Decanniere K, Pelle R, Depoorter J, Brosens E, Parkin DW, Steyaert J. Structure and function of a novel purine specific nucleoside hydrolase from Trypanosoma vivax. J Mol Biol. 2001 Apr 13;307(5):1363-79. PMID:11292348 doi:http://dx.doi.org/10.1006/jmbi.2001.4548
- Degano M, Almo SC, Sacchettini JC, Schramm VL. Trypanosomal nucleoside hydrolase. A novel mechanism from the structure with a transition-state inhibitor. Biochemistry. 1998 May 5;37(18):6277-85. PMID:9572842 doi:http://dx.doi.org/10.1021/bi973012e
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