1yu7
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(New page: 200px<br /><applet load="1yu7" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yu7, resolution 1.5Å" /> '''Crystal Structure of ...)
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Revision as of 04:55, 21 November 2007
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Crystal Structure of the W64Y mutant of Villin Headpiece
Overview
Villin-type headpiece domains are approximately 70 amino acid modular, motifs found at the C terminus of a variety of actin, cytoskeleton-associated proteins. The headpiece domain of villin, a, protein found in the actin bundles of the brush border epithelium, is of, interest both as a compact F-actin binding domain and as a model folded, protein. We have determined the high-resolution crystal structures of, chicken villin headpiece (HP67) at 1.4 A resolution as well as two, mutants, R37A and W64Y, at 1.45 and 1.5 A resolution, respectively., Replacement of R37 causes a 5-fold reduction in F-actin binding affinity, in sedimentation assays. Replacement of W64 results in a much more drastic, reduction in F-actin binding affinity without significant changes in, headpiece structure or stability. The detailed comparison of these crystal, structures with each other and to our previously determined NMR structures, of HP67 and the 35-residue autonomously folding subdomain in villin, headpiece, HP35, provides the details of the headpiece fold and further, defines the F-actin binding site of villin-type headpiece domains.
About this Structure
1YU7 is a Single protein structure of sequence from Gallus gallus. Full crystallographic information is available from OCA.
Reference
High-resolution crystal structures of villin headpiece and mutants with reduced F-actin binding activity., Meng J, Vardar D, Wang Y, Guo HC, Head JF, McKnight CJ, Biochemistry. 2005 Sep 13;44(36):11963-73. PMID:16142894
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