1yz9
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(New page: 200px<br /><applet load="1yz9" size="450" color="white" frame="true" align="right" spinBox="true" caption="1yz9, resolution 2.10Å" /> '''Crystal structure of...)
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Revision as of 05:01, 21 November 2007
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Crystal structure of RNase III mutant E110Q from Aquifex aeolicus complexed with double stranded RNA at 2.1-Angstrom Resolution
Overview
Bacterial ribonuclease III (RNase III) can affect RNA structure and gene, expression in either of two ways: as a processing enzyme that cleaves, double-stranded (ds) RNA, or as a binding protein that binds but does not, cleave dsRNA. We previously proposed a model of the catalytic complex of, RNase III with dsRNA based on three crystal structures, including the, endonuclease domain of RNase III with and without bound metal ions and a, dsRNA binding protein complexed with dsRNA. We also reported a, noncatalytic assembly observed in the crystal structure of an RNase III, mutant, which binds but does not cleave dsRNA, complexed with dsRNA. We, hypothesize that the RNase III*dsRNA complex can exist in two functional, forms, a catalytic complex and a noncatalytic assembly, and that in, between the two forms there may be intermediate states. Here, we present, four crystal structures of RNase III complexed with dsRNA, representing, possible intermediates.
About this Structure
1YZ9 is a Single protein structure of sequence from Aquifex aeolicus with SO4 as ligand. Active as Ribonuclease III, with EC number 3.1.26.3 Full crystallographic information is available from OCA.
Reference
Intermediate states of ribonuclease III in complex with double-stranded RNA., Gan J, Tropea JE, Austin BP, Court DL, Waugh DS, Ji X, Structure. 2005 Oct;13(10):1435-42. PMID:16216575
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