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2go2
From Proteopedia
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{{STRUCTURE_2go2| PDB=2go2 | SCENE= }} | {{STRUCTURE_2go2| PDB=2go2 | SCENE= }} | ||
| - | + | ===Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor=== | |
| - | |||
| - | ==Overview== | ||
| - | A Kunitz-type protease inhibitor (BbKI) found in Bauhinia bauhinioides seeds has been overexpressed in Escherichia coli and crystallized at 293 K using PEG 4000 as the precipitant. X-ray diffraction data have been collected to 1.87 A resolution using an in-house X-ray generator. The crystals of the recombinant protein (rBbKI) belong to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 46.70, b = 64.14, c = 59.24 A. Calculation of the Matthews coefficient suggests the presence of one monomer of rBbKI in the asymmetric unit, with a corresponding solvent content of 51% (VM = 2.5 A3 Da(-1)). Iodinated crystals were prepared and a derivative data set was also collected at 2.1 A resolution. Crystals soaked for a few seconds in a cryogenic solution containing 0.5 M NaI were found to be reasonably isomorphous to the native crystals. Furthermore, the presence of iodide anions could be confirmed in the NaI-derivatized crystal. Data sets from native and derivative crystals are being evaluated for use in crystal structure determination by means of the SIRAS (single isomorphous replacement with anomalous scattering) method. | ||
==About this Structure== | ==About this Structure== | ||
| - | 2GO2 is a | + | 2GO2 is a 1 chain structure of sequence from [http://en.wikipedia.org/wiki/Bauhinia_bauhinioides Bauhinia bauhinioides]. Full crystallographic information is available from [http://oca.weizmann.ac.il/oca-bin/ocashort?id=2GO2 OCA]. |
==Reference== | ==Reference== | ||
| - | + | <ref group="xtra">PMID:16511193</ref><ref group="xtra">PMID:16041066</ref><references group="xtra"/> | |
[[Category: Bauhinia bauhinioides]] | [[Category: Bauhinia bauhinioides]] | ||
| - | [[Category: Single protein]] | ||
[[Category: Garratt, R C.]] | [[Category: Garratt, R C.]] | ||
[[Category: Navarro, M V.A S.]] | [[Category: Navarro, M V.A S.]] | ||
[[Category: Beta-trefoil fold]] | [[Category: Beta-trefoil fold]] | ||
| - | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on | + | |
| + | ''Page seeded by [http://oca.weizmann.ac.il/oca OCA ] on Mon Feb 16 18:57:50 2009'' | ||
Revision as of 16:57, 16 February 2009
Crystal structure of BbKI, a Kunitz-type kallikrein inhibitor
About this Structure
2GO2 is a 1 chain structure of sequence from Bauhinia bauhinioides. Full crystallographic information is available from OCA.
Reference
- Navarro MV, Vierira DF, Nagem RA, de Araujo AP, Oliva ML, Garratt RC. Crystallization and preliminary X-ray analysis of a novel Kunitz-type kallikrein inhibitor from Bauhinia bauhinioides. Acta Crystallogr Sect F Struct Biol Cryst Commun. 2005 Oct 1;61(Pt, 10):910-3. Epub 2005 Sep 30. PMID:16511193 doi:10.1107/S1744309105028496
- Nagem RA, Ambrosio AL, Rojas AL, Navarro MV, Golubev AM, Garratt RC, Polikarpov I. Getting the most out of X-ray home sources. Acta Crystallogr D Biol Crystallogr. 2005 Aug;61(Pt 8):1022-30. Epub 2005, Jul 20. PMID:16041066 doi:10.1107/S0907444905012989
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