This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1z4o
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1z4o" size="450" color="white" frame="true" align="right" spinBox="true" caption="1z4o, resolution 1.90Å" /> '''Structure of beta-ph...)
Next diff →
Revision as of 05:06, 21 November 2007
|
Structure of beta-phosphoglucomutase with inhibitor bound alpha-galactose 1-phosphate
Overview
This communication reports the X-ray crystal structure of the -D-galactose-1-phosphate complex with that of Lactococcus lactis -phosphoglucomutase (-PGM) crystallized in the presence of Mg2+ cofactor and the enzyme-to-phosphorus ratio determined by protein and phosphate analyses of the crystalline complex. The 1:1 ratio determined for this complex was compared to the 1:2 ratio determined for the crystals of -PGM grown in the presence of substrate and Mg2+ cofactor. This result verifies the published structure assignment of this latter complex as the phosphorane adduct formed by covalent bonding between the active site Asp8 carboxylate to the C(1)phosphorus of the -glucose 1,6-bisphosphate ligand and rules out the proposal of a -PGM-glucose-6-phosphate-1-MgF3- complex.
About this Structure
1Z4O is a Single protein structure of sequence from Lactococcus lactis with MG and GL1 as ligands. Active as Beta-phosphoglucomutase, with EC number 5.4.2.6 Full crystallographic information is available from OCA.
Reference
Chemical confirmation of a pentavalent phosphorane in complex with beta-phosphoglucomutase., Tremblay LW, Zhang G, Dai J, Dunaway-Mariano D, Allen KN, J Am Chem Soc. 2005 Apr 20;127(15):5298-9. PMID:15826149
Page seeded by OCA on Wed Nov 21 07:14:03 2007
