1zc8

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Revision as of 05:15, 21 November 2007

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Coordinates of tmRNA, SmpB, EF-Tu and h44 fitted into Cryo-EM map of the 70S ribosome and tmRNA complex

Overview

Bacterial ribosomes stalled on defective messenger RNAs (mRNAs) are, rescued by tmRNA, an approximately 300-nucleotide-long molecule that, functions as both transfer RNA (tRNA) and mRNA. Translation then switches, from the defective message to a short open reading frame on tmRNA that, tags the defective nascent peptide chain for degradation. However, the, mechanism by which tmRNA can enter and move through the ribosome is, unknown. We present a cryo-electron microscopy study at approximately 13, to 15 angstroms of the entry of tmRNA into the ribosome. The structure, reveals how tmRNA could move through the ribosome despite its complicated, topology and also suggests roles for proteins S1 and SmpB in the function, of tmRNA.

About this Structure

1ZC8 is a Protein complex structure of sequences from Thermus thermophilus. Full crystallographic information is available from OCA.

Reference

Visualizing tmRNA entry into a stalled ribosome., Valle M, Gillet R, Kaur S, Henne A, Ramakrishnan V, Frank J, Science. 2003 Apr 4;300(5616):127-30. PMID:12677067

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