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2cig
From Proteopedia
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(New page: 200px<br /> <applet load="2cig" size="450" color="white" frame="true" align="right" spinBox="true" caption="2cig, resolution 1.90Å" /> '''DIHYDROFOLATE REDUC...)
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Revision as of 19:33, 29 October 2007
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DIHYDROFOLATE REDUCTASE FROM MYCOBACTERIUM TUBERCULOSIS INHIBITED BY THE ACYCLIC 4R ISOMER OF INH-NADP A DERIVATIVE OF THE PRODRUG ISONIAZID.
Overview
Isoniazid is a key drug used in the treatment of tuberculosis. Isoniazid, is a pro-drug, which, after activation by the katG-encoded catalase, peroxidase, reacts nonenzymatically with NAD(+) and NADP(+) to generate, several isonicotinoyl adducts of these pyridine nucleotides. One of these, the acyclic 4S isomer of isoniazid-NAD, targets the inhA-encoded enoyl-ACP, reductase, an enzyme essential for mycolic acid biosynthesis in, Mycobacterium tuberculosis. Here we show that the acyclic 4R isomer of, isoniazid-NADP inhibits the M. tuberculosis dihydrofolate reductase, (DHFR), an enzyme essential for nucleic acid synthesis. This biologically, relevant form of the isoniazid adduct is a subnanomolar bisubstrate, inhibitor of M. tuberculosis DHFR. Expression of M. tuberculosis DHFR in, ... [(full description)]
About this Structure
2CIG is a [Single protein] structure of sequence from [Mycobacterium tuberculosis] with SO4, 1DG and GOL as [ligands]. Active as [[1]], with EC number [1.5.1.3]. Full crystallographic information is available from [OCA].
Reference
Mycobacterium tuberculosis dihydrofolate reductase is a target for isoniazid., Argyrou A, Vetting MW, Aladegbami B, Blanchard JS, Nat Struct Mol Biol. 2006 May;13(5):408-13. Epub 2006 Apr 30. PMID:16648861
Page seeded by OCA on Mon Oct 29 21:38:27 2007
