1zgt
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(New page: 200px<br /><applet load="1zgt" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zgt, resolution 1.45Å" /> '''Structure of hydroge...)
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Revision as of 05:19, 21 November 2007
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Structure of hydrogenated rat gamma E crystallin in H2O
Overview
Rat gammaE-crystallin was overexpressed, purified under different, labelling conditions and crystallized and X-ray data were collected at, resolutions between 1.71 and 1.36 A. The structures were determined by, molecular replacement. In these structures, the cd loop of the Greek-key, motif 3, which is the major structural key motif of the two, phase-transition groups of gamma-crystallins, presents a double, conformation. The influence of the perdeuteration on the protein structure, was determined by comparison of the atomic positions and temperature, factors of the different models. The perdeuterated proteins have a similar, structure to their hydrogenated counterparts, but partial or full, deuteration may have some effect on the atomic B-factor values.
About this Structure
1ZGT is a Single protein structure of sequence from Rattus norvegicus with ACT as ligand. Full crystallographic information is available from OCA.
Reference
A comparison of refined X-ray structures of hydrogenated and perdeuterated rat gammaE-crystallin in H2O and D2O., Artero JB, Hartlein M, McSweeney S, Timmins P, Acta Crystallogr D Biol Crystallogr. 2005 Nov;61(Pt 11):1541-9. Epub 2005, Oct 19. PMID:16239733
Page seeded by OCA on Wed Nov 21 07:26:36 2007
