1zlh

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Revision as of 05:24, 21 November 2007

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spinqualitylabelsall models 1zlh, resolution 1.70Å Show:Asymmetric Unit Biological Assembly Drag the structure with the mouse to rotate   Export Animated Image

Crystal structure of the tick carboxypeptidase inhibitor in complex with bovine carboxypeptidase A

Overview

The tick carboxypeptidase inhibitor (TCI) is a proteinaceous inhibitor of, metallo-carboxypeptidases present in the blood-sucking tick Rhipicephalus, bursa. The three-dimensional crystal structures of recombinant TCI bound, to bovine carboxypeptidase A and to human carboxypeptidase B have been, determined and refined at 1.7 A and at 2.0 A resolution, respectively. TCI, consists of two domains that are structurally similar despite the low, degree of sequence homology. The domains, each consisting of a short, alpha-helix followed by a small twisted antiparallel beta-sheet, show a, high level of structural homology to proteins of the beta-defensin-fold, family. TCI anchors to the surface of mammalian carboxypeptidases in a, double-headed manner not previously seen for carboxypeptidase inhibitors:, the last three carboxy-terminal amino acid residues interact with the, active site of the enzyme in a way that mimics substrate binding, and the, N-terminal domain binds to an exosite distinct from the active-site, groove. The structures of these complexes should prove valuable in the, applications of TCI as a thrombolytic drug and as a basis for the design, of novel bivalent carboxypeptidase inhibitors.

About this Structure

1ZLH is a Protein complex structure of sequences from Bos taurus and Rhipicephalus bursa with ZN as ligand. Active as Carboxypeptidase A, with EC number 3.4.17.1 Full crystallographic information is available from OCA.

Reference

The three-dimensional structures of tick carboxypeptidase inhibitor in complex with A/B carboxypeptidases reveal a novel double-headed binding mode., Arolas JL, Popowicz GM, Lorenzo J, Sommerhoff CP, Huber R, Aviles FX, Holak TA, J Mol Biol. 2005 Jul 15;350(3):489-98. PMID:15961103

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