This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1zuf
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /><applet load="1zuf" size="450" color="white" frame="true" align="right" spinBox="true" caption="1zuf" /> '''Solution Structure of DLP-4'''<br /> ==Over...)
Next diff →
Revision as of 05:32, 21 November 2007
|
Solution Structure of DLP-4
Overview
The recent discovery that the natriuretic peptide OvCNPb (Ornithorhynchus, venom C-type natriuretic peptide B) from platypus (Ornithorynchus, anatinus) venom contains a D-amino acid residue suggested that other, D-amino-acid-containing peptides might be present in the venom. In the, present study, we show that DLP-2 (defensin-like peptide-2), a, 42-amino-acid residue polypeptide in the platypus venom, also contains a, D-amino acid residue, D-methionine, at position 2, while DLP-4, which has, an identical amino acid sequence, has all amino acids in the L-form. These, findings were supported further by the detection of isomerase activity in, the platypus gland venom extract that converts DLP-4 into DLP-2. In the, light of this new information, the tertiary structure of DLP-2 was, recalculated using a new structural template with D-Met2. The structure of, DLP-4 was also determined in order to evaluate the effect of a D-amino, acid at position 2 on the structure and possibly to explain the large, retention time difference observed for the two molecules in reverse-phase, HPLC. The solution structures of the DLP-2 and DLP-4 are very similar to, each other and to the earlier reported structure of DLP-2, which assumed, that all amino acids were in the L-form. Our results suggest that the, incorporation of the D-amino acid at position 2 has minimal effect on the, overall fold in solution.
About this Structure
1ZUF is a Single protein structure of sequence from [1]. Full crystallographic information is available from OCA.
Reference
D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties., Torres AM, Tsampazi C, Geraghty DP, Bansal PS, Alewood PF, Kuchel PW, Biochem J. 2005 Oct 15;391(Pt 2):215-20. PMID:16033333
Page seeded by OCA on Wed Nov 21 07:40:04 2007
