This old version of Proteopedia is provided for student assignments while the new version is undergoing repairs. Content and edits done in this old version of Proteopedia after March 1, 2026 will eventually be lost when it is retired in about June of 2026.
Apply for new accounts at the new Proteopedia. Your logins will work in both the old and new versions.
1afk
From Proteopedia
OCA (Talk | contribs)
(New page: 200px<br /> <applet load="1afk" size="450" color="white" frame="true" align="right" spinBox="true" caption="1afk, resolution 1.7Å" /> '''CRYSTAL STRUCTURE OF...)
Next diff →
Revision as of 19:36, 29 October 2007
|
CRYSTAL STRUCTURE OF RIBONUCLEASE A IN COMPLEX WITH 5'-DIPHOSPHOADENOSINE-3'-PHOSPHATE
Overview
High-resolution (1.7 A) crystal structures have been determined for bovine, pancreatic ribonuclease A (RNase A) complexed with 5'-diphosphoadenosine, 3'-phosphate (ppA-3'-p) and 5'-diphosphoadenosine 2'-phosphate (ppA-2'-p), as well as for a native structure refined to 2.0 A. These nucleotide, phosphates are the two most potent inhibitors of RNase A reported so far, with Ki values of 240 and 520 nM, respectively. The binding modes and, conformations of ppA-3'-p and ppA-2'-p were found to differ markedly from, those anticipated on the basis of earlier structures of RNase A complexes., The key difference is that the 5'-beta-phosphate rather than the, 5'-alpha-phosphate of each inhibitor occupies the P1 phosphate binding, site. As a consequence, the ribose moieties of the two nucleotides ... [(full description)]
About this Structure
1AFK is a [Single protein] structure of sequence from [Bos taurus] with PAP as [ligand]. Active as [[1]], with EC number [3.1.27.5]. Full crystallographic information is available from [OCA].
Reference
Crystal structures of ribonuclease A complexes with 5'-diphosphoadenosine 3'-phosphate and 5'-diphosphoadenosine 2'-phosphate at 1.7 A resolution., Leonidas DD, Shapiro R, Irons LI, Russo N, Acharya KR, Biochemistry. 1997 May 6;36(18):5578-88. PMID:9154942
Page seeded by OCA on Mon Oct 29 21:40:54 2007
