2a6u
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(New page: 200px<br /><applet load="2a6u" size="450" color="white" frame="true" align="right" spinBox="true" caption="2a6u, resolution 3.28Å" /> '''pH evolution of tetr...)
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Revision as of 05:49, 21 November 2007
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pH evolution of tetragonal HEWL at 4 degrees Celcius.
Overview
44 samples of tetragonal hen egg-white lysozyme (HEWL) were obtained as a, series of polycrystalline precipitates at 277 K and room temperature in, the pH range between 6.56 and 3.33. The precipitates were investigated by, the collection of high-resolution powder X-ray diffraction data at 295 K, which reveal the tetragonal or orthorhombic forms of lysozyme depending on, the temperature and pH of crystallization. The use of a new robotic sample, changer greatly facilitated these measurements. LeBail analyses of the, powder patterns display a characteristic behaviour for the pH dependence, of the tetragonal unit-cell parameters of HEWL crystallized at both, temperatures. More detailed analysis shows that molecular replacement can, give a suitable starting point for structural refinements, illustrating, that powder data can be sufficient for this approach. Pawley or Rietveld, refinements that fit a single model to four data sets simultaneously from, four samples crystallized at pH values across the range studied benefit, from improved powder data quality via the anisotropic changes in the unit, cell. The Rietveld analysis gave an average structural model with, excellent goodness of fit and stereochemistry.
About this Structure
2A6U is a Single protein structure of sequence from Gallus gallus. Active as Lysozyme, with EC number 3.2.1.17 Full crystallographic information is available from OCA.
Reference
High-throughput phase-diagram mapping via powder diffraction: a case study of HEWL versus pH., Basso S, Fitch AN, Fox GC, Margiolaki I, Wright JP, Acta Crystallogr D Biol Crystallogr. 2005 Dec;61(Pt 12):1612-25. Epub 2005, Nov 19. PMID:16301795
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